Phytoenzymes of plant origin 1. Exogenous metabolic plant enzymes 2. Exogenous autolytic plant enzymes a.
Discussion The Discussion should be written after the Results section so that you have a good idea of what the experiment has demonstrated. The discussion section should definitely have a statement of your expected findings.
Pechenik, 86 This should include your hypothesis and a brief statement about why these types of results are expected. There should also be a comparison of how your actual results related to your expected findings. Pechenik, 86 Here, you should state whether or not your results supported or didn't support your hypothesis.
In addition, the degree to which the evidence supported your hypothesis should be stated. For example, were the results completely supportive, or were there variances?
There should be an explanation of unexpected results. Pechenik, 86 When looking for possible explanations, consider the following: Was the equipment used adequate for the task? Was the experimental design valid? Were the working assumptions made correct? A common mistake that many writers make is to blame themselves for the unexpected results.
Unless you actually made a mistake following the methods of the experiment, and could not go back and correct it, do not make up such errors to explain the variances you observe.
Think about and analyze the methods and equipment you used. Could something different have been done to obtain better results? Another possibility to consider is if the experiment was conducted under factors that were considerably different from those described in the manual. Be sure to include ideas on how to test these explanations.
For example, if there is a problem with the methods, maybe the experiment should be reproduced with an added step. Also mention what kinds of experiments still need to be conducted in order to obtain more information.
The results of the first experiment supported the hypothesis that the rate of conversion of the substrate would increase with increased amounts of enzyme. We observed that Tube 2, which had the highest concentration of enzyme, catecholase, also had the highest absorbance level.
Since absorbance is used as a measure of reaction, the greatest rate of conversion of catechol and oxygen to benzoquinone was seen in Tube 2.
The high ratio of enzyme to substrate caused the absorbance to grow rapidly and then level off see Figure 1.
The tubes with lower concentrations of enzyme had lower rates of conversion, as expected. However, there were some unexpected results in Tube 2. Between the time of around 6 minutes to 8 minutes there was decrease in the absorbance. One explanation of this observation is that the settling of the substrate to the bottom of the test tube caused the enzyme to become less efficient since it could not attack the substrate as well.
The settling reduced the surface area of the substrate that could be attacked by the enzyme. The tube was inverted and the substrate was stirred up, which caused a rise in the absorbance.
Further experiments, involving the constant stirring of the solution, could be performed to test this possibility. The folding and combination of polypeptide chains forms the specific, three dimensional shape of an enzyme.
This shape is extremely important to the enzyme's catalyzing efficiency and many environmental conditions can affect the shape of enzymes and thus their efficiency.
A range of pH values exists for all enzymes, between which they reach their maximum catalyzing action. This range is usually between a pH of The results we obtained supported this assumption for the catecholase enzyme.
The catecholase samples in tubes 3 and 4 had similar absorbance rates and, therefore, similar enzyme activities. However, the pH of 4 in tube 2 corresponded to low absorbance and low activity of the enzyme in that tube.
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BIO10 Lab 3 Enzymes and pH 31 6. Mix the rennin and milk to start the enzyme reactions. a. With a clean squeeze bulb, put 3 drops of rennin solution from the “cold” tube of rennin into the “cold” milk. The Dissolution Discussion Group (DDG) Web site (sponsored by Agilent, Inc.) features an active dissolution bulletin board where dissolution professionals can discuss dissolution automation, autosampling, chemical and mechanical calibration, equipment, accessories, methods, analysis, calculation, regulation, and dissolution guidelines.
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Frequently Asked Questions (23 total) adapted excerpt from book 'Enzymes for Autism and other Neurological Conditions'.
copyright October last updated 1. What are enzymes?